(c) A disposable filter system with a 0.22 μm cellulose acetate membrane filter. The filtrate is collected in a test tube or other suitable container. Samples are placed in the syringe and pushed through the filter. In the photo on the left, the filter is attached to a syringe. The photo on the right shows the membrane filter in its casing. Upon spinning the unit at \(2000 \times g-5000 \times g\), the filtrate collects in the bottom reservoir and the retentate remains in the upper reservoir. The sample is placed in the upper reservoir and the unit is placed in a centrifuge. The membrane has a nominal molecular weight cut-off of \(1 \times 10^6\) g/mol. (a) A centrifugal filter for concentrating and desalting macromolecular solutions. Examples of three types of membrane filters for separating analytes and interferents. For applications of gravity filtration and suction filtration in gravimetric methods of analysis, see Chapter 8. Provides information about three types of membrane filters. A membrane filter is the method of choice for particulates that are too small to be retained by filter paper. Gravity filtration and suction filtration using filter paper are techniques with which you should already be familiar. The solution that passes through the filter is called the filtrate, and the material retained by the filter is the retentate. In a filtration we separate a particulate interferent from soluble analytes using a filter with a pore size that will retain the interferent. Examples of size-based separations include filtration, dialysis, and size-exclusion. To accomplish the separation we use a porous medium through which only the analyte or the interferent can pass. Size is the simplest physical property we can exploit in a separation.
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